Low-conductance chloride channel from crayfish skeletal muscle incorporated into planar lipid bilayers.

Ryanodine receptor purified from crayfish skeletal muscle.

The ryanodine receptor was isolated from the sarcoplasmic reticulum of crayfish skeletal muscle. Ryanodine binding to the native fraction was measured by Scatchard analysis and values of 60 nmol/l and 9 pmol/mg were obtained for KD and Bmax respectively. The identity of purified receptor was confirmed by electron microscopy, electrophoresis and incorporation into planar lipid bilayers. At least...

DHP-sensitive Ca2+ channels from crayfish skeletal muscle T-tubules incorporated into planar lipid bilayers.

Characterization of biophysical properties of single chloride channel in rat brain mitochondrial inner membrane by channel incorporation into bilayer lipid membrane

Introduction: Recent studies have shown the presence of Cl- channels in heart and liver mitochondrial membranes. In this work, we have characterized the functional profile of a Cl- channel from rat brain mitochondria. Methods: After removing and homogenizing the rat brain, the supernatant was separately centrifuged in MSEdigitonin, H2O and Na2CO3 and mitochondrial inner membrane vesicles wer...

Two types of potassium channels from the internal membrane system of the crayfish muscle incorporated into planar lipid bilayers.

Potassium channels constitute the most diverse and widespread class of ion channels known. K channels have been found in all cells which display action potentials mediated by sodium or calcium ions. The different types of K channels are very often present in the same cells which shows, that their diversity is not associated with the cell type (for review see Latorre and Miller 1983; Rudy 1988)....

Ethanol potentiation of calcium-activated potassium channels reconstituted into planar lipid bilayers.

We examined the actions of ethanol on the single channel properties of large conductance Ca2+-activated K+ (BK) channels isolated from skeletal muscle T-tubule membranes and incorporated into planar lipid bilayer membranes. We have taken advantage of this preparation, because it lacks most elements of cellular complexity, including cytoplasmic constituents and complex membrane lipid composition...